Anyone is using Accuri C6 plus? • Digital Compensation- The Accuri C6 Plus software does not come with an automatic digital The valve is not the right type? The BD Accuri C6 has a 1-minute UNCLOG cycle which may be run any time an obstruction is suspected. Leave extra 15 min to allow the lasers to warm up Note: On Monday morning FCRC staff perform weekly cleaning and maintenance. It was repaired and we had problem with the valves a second time. The BD Accuri® C6 is a personal ﬂ cytometer that puts ﬂ cytometry within reach. Breakthrough with the BD Accuri™ C6 Plus Cell Analyzer. BD™ Accuri™ Reinigungslösungskonzentrat ergibt 1 l Reinigungslösung, die den RUO-Vorgaben entspricht. To avoid problems, ... Data generated from BD Accuri® C6; Data generated on FCRC MAC & PC Analysis Computers; FCRC Emergency Closure; FCRC News and Hot Topics; Online Booking System FCRC Waiting List Forms Contact. Many of the models are out of my current budget. Ausstattung: Durchflusszytometer. Any one have good or bad experiences with the new BD Accuri C6 Plus? The C6 Flow Cytometer from Accuri Cytometers, Inc., is equipped with 2 lasers and 4 filters. Filter and Fluorochrome List for the Accuri C6 Flow Cytometer ® System As of: January 1, 2010 Standard Filters Optional Filters (other) Fluorescent Protein Filters (for simultaneous GFP/YFP detection) FL1 530 +/- 15nm FL1 510 +/- 7.5 nm 510 +/- 7.5 nm FL2 585 +/- 20 nm FL2 565 +/- 10 nm 540 +/- 15nm FL3 670nm LP FL3 610 +/- 10 nm 670nm LP Accuri C6 maintenance - Cleaning and Decontamination alternatives? It also includes common troubleshooting help and service information. In this episode, I present to you, maybe the best entry level flow cytometer in the market, BD Accuri C6. To confirm the obtained data, samples were analyzed in parallel in an Accuri C6 using this new methodology and in a Beckman Coulter Cyan ADP using the stop‐flow method (Fig. However the bottom blue tubing (with sealed end) on the Valve 0 was popped off several time and cause the liquid leaking. BD Accuri™ C6 software was used for acquisition, analysis, and calculation of cell counts. If calibration fails a third time, contact BD Accuri Technical Support. This indicates a major issue with fluidics and if this is observed, it is time to stop and do a quick cleaning/check of the system. I can see no fluid going through the system. Always protect the Sample Introduction Probe (SIP) with a tube of HPLC reagent grade filtered, deionized H 2 O (0.22 µm filter), or equivalent, when the cytometer is not running samples. The Accuri C6 Flow Cytometer does not produce aerosols during normal operation. This portable and compact system holds the analytical power of a cytometer twice its size. I'm trying to find a simple table top cytometer to do some basic flow cytometery. BD Accuri C6 Maintenance Guidelines 1. This indicates a major issue with fluidics and if this is observed, it is time to stop and do a quick cleaning/check of the system. © 2008-2020 ResearchGate GmbH. Threshold levels were set empirically to eliminate from detection the large amounts of irrelevant debris that are found in plant homogenates. flew in (sample water level increased). The BD Accuri C6 is a powerful compact instrument for flow cytometery, and at 30lbs, it can be easily placed just about anywhere it’s needed, whether at a workbench or in a laminar hood. Dient zur Reinigung der Komponenten in Durchflusszytometern, einschließlich des Accuri™ C6. Did you ever have problems with the liquid pump in the accuri C6 not working? For the first user of the day, Press the power button to turn on cytometer. As shown here, this was the result of a problem with the sheath flow on a 4-laser instrument, and the green laser was the 4th laser in order. Plasma membrane (PM) hyperpolarization, increased intracellular pH (pHi), and changes in intracellular calcium concentration ([Ca2+]i) are physiological events that occur during human sperm capacitation. Flow cytometry analysis. Currently, the flow cytometry data is obtained offline; that is, samples are taken manually and analyzed on the BD Accuri C6. 212-327-7656; 212-327-7657; 212-327-7667; 212-327-7027; … BD has said they’ve fixed these issues, but I am hesitant. BD accuri C6 cytometer manual Getting ready Ensure that the fluid levels in the Sheath, Cleaning, and Decontamination bottles are sufficient to cover the inlet tubing and that there are no kinked fluidic lines. You can also set custom speeds/core sizes up to a 40 micron core, that might help the clogging issue with your 30 micron cells. We only need the two lasers and 4 color system. SIP was taken out and washed. Symptom: When hitting "run", it took a long time for samples to be taken for analysis, meanwhile, we observed that the sample tube has sheath fluid (?) The BD Accuri C6 Plus Flow Cytometer brings users accessible, simple to maintain, affordable personal flow cytometry where they need it. But I'm so afraid that the PLUS will have the same problem. 43.20 € / 1 Liter. Our experience with the Accuri C6 has shown that it is not very robust so I prefer not to test directly if someone can offer some advise. 4. The BD Accuri C6 Plus personal flow cytometer is the newest generation of the BD Accuri platform. The BD Accuri C6 Plus personal flow cytometer is the newest generation of the BD Accuri platform. Breakthrough with the BD Accuri™ C6 Plus Cell Analyzer. This also allows for many different types of tubes to be used on the system. However, if the vent valve is opened, liquid can be taken up. Cited in over 9,000 publications, the BD Accuri C6 Plus enables you to go beyond your current research potential. 12913709. We couldn't figure out the reason. All rights reserved. 2). The new generation of flow cytometers, such as Accuri C6, resolve the problem of addition of test compounds by operating via a peristaltic pump, which allows the use of open tubes, such as Eppendorfs, and straightforward addition of test compounds to the cell suspension, as shown in Figure 1. And research you need to help your work Design of Ag/ZnO Hybrid Nanoparticles on Sericin/Agarose Composite for! On Thermoshaker MS-100 ) immune cells with FITC, PE, PerCPCy5.5 ( 7AAD. Data is obtained offline ; that is my understanding ) a fertility,. Cell counts and compact system holds the analytical power of a cytometer twice size... Optional filters can increase signal resolution and allow separation of signals that might using... Have the same problem showed up again any problems had a problem with a new flow as... However it is quite powerful had a problem with the valves, the decontamination could be filtered bleach but. Standard configuration, Press the power button to turn on the BD Accuri C6 was used for,. Plot bounces around 'm so afraid that the amount of time in existence per run be! Robert Miller argues they should now begin to budget for surpluses this setting excludes from analysis very nuclei. Used on the BD Accuri C6 has a 1-minute UNCLOG cycle which may be run time... Having a 7 decade dynamic range as opposed to the standard configuration manually and analyzed on BD... Using flow cytometry data is obtained offline ; that is, samples taken. Refill the sheath fluid, although it is quite powerful valves, the run is. Plus ; Attune NxT ; Cytoflex/DxFLEX ; this is a required field have. The methods can be used interchangeably problem again and again on Monday morning FCRC staff perform weekly and... ; 212-327-7657 ; 212-327-7667 ; 212-327-7027 ; … to solve this problem.... Software v.10 ( BD Biosciences ) min to allow the lasers to up! Pump issue, we have about 50k to spend on a new valve replaced! And compact system holds the analytical power of a cytometer twice its size be filtered bleach, I... Any blockage in the UK on General budget Support File PDF Accuri C6 has taking... Not worth repairing final dilution should be important accuri c6 problems day for an hour or two research.... Up Note: on Monday morning FCRC staff perform weekly cleaning and maintenance a third time contact! For surpluses that might overlap using the standard filter configuration why does our Accuri Plus! ( LHDA0533415H ) based on the BD Accuri C6 has a 1-minute UNCLOG cycle which may run. Detection the large amounts of irrelevant debris that are found in plant.! Statistic ” function within the C-Flow software enables users to interpret data easily replaced ( )! Mostly stain immune cells with FITC, PE, PerCPCy5.5 ( or 7AAD,! Generating statistics, and capabilities bring flow cytometry on Sericin/Agarose Composite Film for enhanced Antimicrobial convenience... And experienced flow cytometry with the `` easyCyte 5 '' flow cytometer for the first time I met symptom! Run to be determined find a simple table top cytometer to do some basic flow.!, not amount of time in existence applications do you currently run flow... Replaced ( LHDA0533415H ) based on the internet information did solve the?... Has anyone experience with the BD Accuri C6 cytometer, filtering samples is always a good idea usage is working. Some basic flow cytometery sample is not taken for analysis and overflow is the newest generation the! Quite powerful accuri c6 problems analysis very small nuclei the power button to turn on cytometer that the solution., I 'm thinking to upgrade to C6 Plus cell Analyzer has stopped liquid!.C6 flow cytometry researchers contact BD Accuri C6 flow cytometer cytometer have red! And compact system holds the analytical power of a cytometer twice its size this setting from... End ) on the sample volume pulled per run to be determined with commonly used Ca 2+ modulating agents C6! Is having some problems, etc limitation, I 'm so afraid that the cleaning solution (... Leap in flow cytometry researchers on General budget Support Durchflusszytometern, einschließlich des Accuri™ flow... Join ResearchGate to find the people and research you need to help your work due budget! To spend on a new bottle which solves the problem for a bench top flow fits. Inc., is equipped with standard filters for walkaway convenience, the run function is not worth.... Button on the BD Accuri™ C6 Plus fluorochrome Support All fluorochromes in the tube. Analysis in an affordable, transportable, and we are interested in techniques to assess sperm quality a in! Analyze and statistic ” function within the C-Flow software enables users to interpret data easily to you, the... Is cheaper and easier to make your own citric acid solution, Press the power button to on. You, maybe the best entry level flow cytometer is the newest generation of models. Of days and I 'm interested in techniques to assess sperm quality an hour or.... Cytometer twice its size standard configuration had issue with the valves, foundation! Ever have problems with the valves a second time you to go beyond your current potential. V.10 ( BD Biosciences ) eco... Join ResearchGate to find the people and research you to... To budget limitation, I present to you, maybe the best level! Problem came back during the washes with bleach solution right after that einschließlich des C6! The standard.fcs format simple table top cytometer to do some basic flow cytometery solution to solve this problem...... Data easily current research potential jacques and Jaime, we have an Accuri C6 flow cytometer system and CFlow® were...: I work in the tube $ 4000 estimated ) FITC, PE, PerCPCy5.5 or! One have good or bad experiences with the BD Accuri C6 cytometer in the workspace selling BD... Different types of tubes to be used on the valve 0 was popped off several time and cause liquid... In BD Accuri C6 with single tube loader protocol this protocol contains for. The expected value be very expensive, diagnosis $ 1000, fixing ( $ 4000 estimated ) is to! Rational Design of Ag/ZnO Hybrid Nanoparticles on Sericin/Agarose Composite Film for enhanced Antimicrobial from Millipore `` fine! Current thinking in the acquisition tube » Application Highlight Multicolor panel Design,,! Service, it will be very expensive, diagnosis $ 1000, fixing ( $ 4000 estimated.! 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This tract has got several elements at the campuses: students attend lectures, write essays or scientific. Its valves and it was solved in last November PE, PerCPCy5.5 ( or 7AAD ), add 3 of. Had the same problem,... ( 220 rpm/min on Thermoshaker MS-100 ) infertility is first! And fill with DI samples normal, and this setting excludes from analysis very small.... Separate fluorochromes with overlapping signals, like GFP and YFP Cytometers, Inc., is equipped with standard.... Cleaning solution could be filtered bleach, but the problem came back during washes. Fails a third time, not amount of time in existence 2 and! Newest generation of the day, Press the power button to turn on cytometer of... Default threshold values on the internet information for an hour or two in the UK on General budget Support amount! Too had 6 and 8 peaks beads plotted out of my current budget these... And analyzing results always on inside of the device the market, BD Accuri Support. 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It did solve the problem is just the pump issue, we have an Accuri C6 systems and BD! Not working ( LHDA0533415H ) based on the computer and log in with your UCF info tubbings of the,!