BD Biosciences offers reagents and kits that facilitate cell cycle analysis both in vitro and ex vivo. The BD Accuri C6 Plus personal flow cytometer is the newest generation of the BD Accuri platform. C6 Configuration: The C6 contains two collinear lasers and is arranged in such a way that four detectors all simultaneously measure light scatter. Using the BD FastImmune IFN-γ/CD69/CD8/CD3 Kit (Cat. 488 nm (rated at 20,000 hr life) 640 nm (rated at 20,000 hr life) Laser Profile. These cookies are used in order to collect information regarding your browsing habits and profiling your center of interest with the aim of showing you advertisements and BD communications when they are relevant to your personal interests. La vitesse de flux maximum est de 10 000 évènements par seconde. SYBR® is a registered trademark of Life Technologies Corporation. Adding propidium iodide (PI) to the protocol can further discriminate bacteria with disrupted vs intact membranes. Please review our cookies policy and configure your cookies for your experience. Accuri C6 is an analyzer equipped with two lasers and 4 color detectors and it has capabilities similar to FACSCalibur. Simultaneous detection of multiple fluorescent proteins, Detection of green and yellow fluorescent proteins. Students obtain time zero blood differentials on mice by blood smear microscopy. Cells were stimulated with PMA+Ionomycin for several hours before collecting culture supernatants. Human PBMCs were cultured for several days with plate-bound anti-CD3, soluble anti-CD27, IL-2, and IL-4. Access to training videos presented by FCR staff are here. The standard optical filters in the BD Accuri C6 Plus are optimized to detect common fluorochromes such as FITC/BB515, PE, PerCP, and APC. 653126), researchers can operate the system in 2-blue/2-red and 4-blue configurations, which significantly Even novice flow cytometry users find it so easy to learn and use that most can collect and analyze data in less than an hour. Access to training videos presented by FCR staff are here. 561527) and stained with BD Pharmingen™ Mouse Anti-Human CD24 PE and BD Pharmingen™ Mouse Anti-Human CD44 APC (Cat. Model: BD Accuri C6. Finally, it can measure several cytokines simultaneously for an individual cell. But stresses such as aeration, starvation, and changes in oxygen tension, glucose concentration, and pH can reduce product quantity and quality. The innovative design of the Accuri C6 Flow Cytometer System combines usability and versatility. With 2 lasers: 488 blue and 635 Red, and 4 parameters. This option significantly expands the fluorochrome combinations you can analyze. It is equipped with an argon blue laser (488 nm) that can count and distinguish between groups of marine pico-phytoplankton and heterotrophic prokaryotes below 5 µm. A primary challenge of stem cell research is the inherent heterogeneity of the cell cultures. All three species have high-intensity chlorophyll fluorescence (x-axis, FL3 670 LP optical filter), which varies from a median channel value of 3 x 10. The cells were harvested and stained according to the kit instructions, acquired on a BD Accuri C6 using the kit template, and analyzed using BD Accuri C6 software. Compact and rugged, they can be transported into labs and processing facilities, and even into the field. You can analyze cell cycle and aneuploidy or trace signaling cascades across multiple subpopulations. The BD Accuri incorporates efficient collection optics and two lasers(488-blue and 633 red) to analyze up to four colors. Researchers who study, isolate, and culture mesenchymal stromal cells (MSCs) must continually monitor and verify their purity and multipotency. With its compact 11 x 14.75 x 16.5-inch footprint, light weight of 30 lb, and operational simplicity, the BD Accuri C6 Plus supports a wide array of applications including immunology, cell and cancer biology, plant and microbiology, and industrial applications. For Research Use Only. Because intracellular analysis requires permeabilization, surface marker analysis is essential when researchers want to isolate live cell populations for further analysis. These cookies collect information on how a website is used, they do not store personal information. Detecting these events has broad application in the fields of cell biology, cancer research, and developmental biology. Passage en tube, microtube ou eppendorf . Cell cycle phases are clearly distinguished in plots showing. The Accuri has no adjustable PMT so there are no voltages to set, making it very straight forward to use. How you intend to take your Flowcytometry work forward in the years >to come > > >We do not have an Accuri, but an Accuri C6 is a 4 color (2 lasers) >instrument, with some researchers claiming it to be an excellent user >friendly machine, simple to use especially for repetitive experiments, >inexpensive, and easy to train novices on. Training can be requested via PPMS. Continuous, gap-free recording of intracellular calcium levels on the BD Accuri C6, Interaction of nanoparticles with mammalian cells, Continuous sampling at varying temperatures. Analytical and performance characteristics are not established. As expected, MDA-MB-231 cells (upper plots) expressed a cancer stem cell phenotype (CD44. The productivity of phytoplankton and cyanobacteria species responsible for harmful algal blooms is of critical concern. Cell signaling networks are critically involved in the regulation of cellular function. Flow cytometry can rapidly and accurately immunophenotype many kinds of cells, including lymphoid tissue (thymus, spleen, and lymph nodes), digested solid tissues, and blood. A high correlation between PI-DNA emission in the FL2 and FL3 channels places the nuclear events in a narrow diagonal region from which it is easy to exclude fragments and debris. Open tubes, such as Eppendorf® tubes, allow convenient addition of test compounds to the cell suspension without interrupting sampling. BD Accuri systems can help you quickly examine reporter gene transfection efficiency and cell population heterogeneity—right from your benchtop. Filter, laser, and configuration options provide added flexibility. Flow cytometry can provide a rapid and efficient way of quantifying DNA content using dyes such as propidium iodide (PI). The BD CSampler Plus adds a minimal footprint to the BD Accuri C6 Plus, about three feet square for the pair, keeping your benchtop free for other uses. In three environmental water samples, autotrophic phytoplankton were identified based on detection of chlorophyll a and b, and characterized as blue-green algae (cyanobacteria) or red algae based on Phycoerythrin and Phycocyanin fluorescence. The BD Accrui C6 Cytometer -The cytometer has 2 lasers: -Blue 488nm laser (3 colors) -Red 640 (1 color) -Unlike most cytometers you will encounter, the Accuri does not all you to manually adjust your voltage settings -Instead, the software uses a larger logarithmic scale and auto-fits events onto the plots for you SYBR® Green and propidium iodide (PI) were used to discriminate live vs dead, Whole blood was analyzed for the expression of CD3 and CD19, two markers expressed differentially on lymphocytes and B cells, respectively. The instrument, therefore, may pose certain hazards associated with these lasers, if misused. Post- acquisition compensation can be performed using Accuri C6 software or third-party applications. Screw in the other two mounting bolts. Light scatter data can reveal basic information about microbes' size, shape, and surface features, while fluorescent stains can assess microbes' cell viability, metabolic activity, and concentration. In essence, BD CBA is equivalent to running up to 30 ELISAs at once using flow cytometry. Deciphering the cell surface proteome enables researchers to define strategies for the analysis and isolation of targeted cells from heterogeneous populations for functional studies, drug screening, and in vivo animal studies. On the Accuri C6, all compensation is performed manually. 661085). Using continuous-flow sampling on the BD Accuri C6, fluorescent nanoparticles were successively added to mammalian cells after 1 minute and 10 minutes and measured as the increase in fluorescence in FL1 (585/40). The BD Accuri™ C6 Plus is equipped with a blue and red laser, two light scatter detectors, and four fluorescence detectors with optical filters optimized for the detection of many popular fluorochromes, including FITC, PE, PerCP-Cy™5.5, and APC, as well as newer polymer dyes such as BD Horizon Brilliant™ Blue 515. Human blood cells were stained and analyzed on the BD Accuri C6. Unless otherwise noted, BD, the BD Logo and all other trademarks are property of Becton, Dickinson and Company. 562574) and stained with PE-conjugated anti-human BD Pharmingen™ FoxP3 monoclonal antibody (Cat. The C6 Flow Cytometer from Accuri Cytometers, Inc., is equipped with 2 lasers and 4 filters. Logiciel d'acquisition : BD CSampler (Manuel d'utilisation).. La configuration optique est modifiable en fonction de vos besoins et dans la mesure où les miroirs dichroïques et … Comparative cytograms of Fluo-4 fluorescence of C6 glioma cells over time, showing the effects of adding control and test compounds (ionophore A23187, ethanol, and thapsigargin). All reagents and kits are compatible with both BD Accuri™ C6 Plus and Accuri™ C6 flow cytometer systems. Students collect peripheral blood from mice and perform peritoneal lavage to obtain infiltrating cells. Figure 7 Figure 8 Figure 9 7. Not for use in diagnostic or therapeutic procedures. Cellular processes and events are probabilistic and take place in a context of complex varying environments, which they influence and which influence them. Chlorophyll autofluorescence (measured in FL3) and forward scatter (FSC) were used to characterize and enumerate phytoplankton in water inflows and outflows of an oyster nursery over the course of a day. BD Lyoplate™ screening panels can help you discover additional surface markers that characterize cells of interest. In one experimental condition, cells were stimulated with PMA+Ionomycin for several hours before harvesting. Researchers at Eawag Aquatic Research in Zurich, Switzerland, have developed a standard flow cytometric staining protocol for BD Accuri flow cytometers to discriminate bacteria from debris in drinking water samples. Exporting Data Among the array of applications that facilitate immunology and stem cell studies, multicolor flow cytometry is preeminent. BD Accuri C6 Plus software files can be exported in FCS 3.1 format for seamless data import into flow cytometry analysis programs such as FCS Express™ software and FlowJo™ software. NOTE: This update does not impact the BD Accuri™ C6 Plus. Staining the samples with SYBR® Green I allows efficient analysis of the total bacterial cell concentration. Not for use in diagnostic or therapeutic procedures. Brand BD Accuri™ Contents Includes Activation Key and Optical Filters: 780/60, 610/20, 630/30. Cell signaling analysis using BD Phosflow reagents. Cells were then stained with 100 nM of BD Pharmingen™ MitoStatus TMRE (Cat. 6. The dashed line indicates the passage trigger point. BD Accuri C6 Flow Cytometer Specifications. The BD Accuri C6 Plus Flow Cytometer brings users accessible, simple to maintain, affordable personal flow cytometry where they need it. Propidium iodide (PI) is impermeable to healthy cells with intact membranes, but permeates cells with compromised membranes such as dead cells. Accurate cell concentrations are essential in many research applications, including enumerating leucocytes, B cells, T cells, and platelets in human blood, measuring microorganism concentrations in purified water, and determining the viability of cultured cell lines. Flow cytometry offers a powerful and effective method for screening microalgal cultures, and BD Accuri systems are especially well suited to the task. Compact and easy to use, the BD Accuri is a highly sensitive tool, providing the investigator with the technological capabilities crucial for sophisticated cellular and molecular work in areas such as cell cycle, cell function, multicolor analysis, and immune function. The BD Accuri C6 Plus can examine post-translational modifications of cell signaling proteins. X-axis values represent direct-volume measurements, while y-axis values were calculated based on counting beads. Bioprocess engineers can use the resulting real-time, online bioreactor data for pinpoint process control. (ni en taille/structure, ni pour les fluorescences). When used in combination in the kit, these dyes provide a rapid, simple method to distinguish live, dead, and injured bacteria, yeast, or eukaryotic cells. Compensation settings remove artifacts of fluorescence spillover on the BD Accuri C6 Plus. So the choice should be addressed by the kind of flow experiments you plan to do. A new method, called continuous or “real-time” flow cytometry, involves adding an agent to a sample while simultaneously analyzing it. The cycle may be repeated until fluid is observed in the tube. 11. No. All reagents and kits are compatible with both BD Accuri C6 Plus and BD Accuri C6 systems. Cells were gated on light scatter properties of H9 hESCs and analyzed for expression of key pluripotency surface markers and transcription factors using BD Accuri C6 software. Events are gated on high Fluo-4 fluorescence to exclude fragments. Two additional features can further simplify your research: BD Biosciences offers a range of cost-effective cell biology kits ideally suited for the BD Accuri that include needed reagents and buffers to assess apoptosis, cell cycle and proliferation, and DNA damage. The instructor injects mice with thioglycollate to induce peritonitis. Although not required, these products are manufactured in accordance with Good Manufacturing Practices. Beyond simple counting, flow cytometry has also been instrumental in studying the factors that cause wide individual variations in platelet activation, aggregation, and thrombus formation. This means that they can count platelets without the need for a hematology analyzer. BD CSampler Plus automatic sampling accessory. The BD CSampler adds minimal footprint to the BD Accuri C6, about three feet square for the pair, keeping the benchtop free for other uses. Microsoft and Excel are registered trademarks of Microsoft Corporation. K562 leukemia cells (incorporating the Philadelphia translocation) were cultured, stained with the BD Cycletest™ Plus DNA Reagent Kit (Cat. Laser Excitation. The following sections and resources illustrate the rich data you can generate by using BD Accuri systems for cell and cancer biology applications. The BD Accuri™ C6 Flow Cytometer An affordable, full-featured, easy-to-use flow cytometer. Free BD Accuri software templates simplify data collection and analysis. The cells were then fixed, lysed and permeabilized using the BD Pharmingen™ Human Transcription Factor Buffer Set (Cat. ACCURI C6 Tweeter ... NOUVEAU module de changement de configuration des lasers: voir les différentes configurations possibles (et la liste des fluorochromes associés) Pas de réglages de PMT nécessaire! Flow cytometry can rapidly quantitate bacteria and discriminate them from debris. Accuri C6 is an analyzer equipped with two lasers and 4 color detectors and it has capabilities similar to FACSCalibur. BD Accuri systems also interface smoothly with automated bioreactor sampling systems so that samples can be taken continuously and analyzed automatically. This feature speeds and simplifies cell analysis by calculating cell concentrations (per unit sample volume) automatically. 560751), purified human peripheral blood mononuclear cells (PBMCs) were stimulated with PMA/Ionomycin (at 50 ng/mL and 1 µg/mL, respectively) in the presence of monensin (BD GolgiStop) protein transport inhibitor (provided in the kit or Cat. Alexa Fluor® is a registered trademark of Life Technologies Corporation. The other tabs contain customizable tools for data analysis, statistics, and batch analysis. To increase signal resolution, or to separate fluorochromes with overlapping signals, a choice of optional, user-interchangeable filters is available. All reagents and kits are compatible with both the BD Accuri C6 Plus and BD Accuri C6 flow cytometer systems. Allows for four blue detectors, or two … Whole blood was stained with fluorescent antibodies to CD3, CD56, CD14, and CD19 and acquired and analyzed on a BD Accuri C6 Plus. Microbe counts and concentrations can be calculated directly and automatically from BD Accuri™ software statistics tables without the addition of counting beads. Open tubes, such as Eppendorf tubes, allow convenient addition of test compounds to the cell suspension without interrupting sampling. The Accuri has no adjustable PMT so there are no voltages to set, making it very straight forward to use. Take advantage of our special offer on the new BD Accuri C6 Plus system. No. Human peripheral blood mononuclear cells (PBMCs) were cultured for several days with plate-bound anti-CD3, soluble anti-CD27, IL-2, and IL-4. In addition to apoptosis, cell cycle, and proliferation studies, immunophenotyping is essential for understanding the cellular ecology of cancer cells. Data was acquired on a BD Accuri C6 and analyzed using BD Accuri C6 software. Because anthropogenic change is driving many species extinct each year, a global molecular and genomic census of angiosperms is needed. No. By installing the optional Selectable Laser Module, you can operate the system in 2-blue/2-red and 4-blue configurations. 1. The real-time method can measure almost any dynamic process and is broadly applicable in cell biology and many other fields of research. Row 1: Triggering on the forward scatter signal of lake water samples does not allow resolution of autofluorescent from non-fluorescent species and debris. © 2020 BD. This portable and compact system holds the analytical power of a cytometer twice its size. In the first two interviews below, researchers describe how they use BD Accuri systems to detect foodborne pathogens and monitor fermentations. Thiazole orange (TO) is a cell-permeant dye that labels both live and dead cells, enabling discrimination of cells from background electronic noise or debris. *Install the BD Accuri C6 Software. Free BD Accuri software templates simplify data collection and analysis. The system accurately monitors the sample volume pulled per run, and can calculate absolute counts or sample concentration per µL without the use of counting beads. 8. © 2020 BD. 555029) protein transport inhibitor. BD Accuri C6 Plus flow cytometer. The BD Accuri C6 is the newest generation of desktop flow cytometers. Bottom row: Control cells cultured with EtOH. 561527), stained according to kit instructions, and acquired on a BD Accuri C6 flow cytometer using the kit template. Enhanced sensitivity, reliability, and capabilities bring flow cytometry even more within reach for new and experienced flow cytometry researchers. 561438) according to the kit procedure, acquired on a BD Accuri C6 flow cytometer using the kit template, and analyzed using BD Accuri C6 software. First, fixed-voltage detectors simplify data collection and reduce the potential for data loss due to signal over- or under-amplification. Two lasers and six detectors. BD Accuri™ C6: 2 Lasers system (2B/2R) 4: BD Accuri™ C6 SORP: 2 Lasers system (2B/2UV) 4: MACSQuant® Analyzer (2 units) 3 Lasers system (4B/2R/1V) 7: Cytek™ Aurora: 4 Lasers system (14B/8R/16V/10YG) 48: Sorter Instrument configuration No.